TIGR Microarray Protocols
and Standard Operating Procedures

Laboratory Protocols
The following laboratory protocols, developed at TIGR, are available in Adobe PDF format.

• Poly A+ RNA Isolation from Total RNA via Dynabeads® Oligo (dT)25
  
• Template Miniprepping
  
• RT-PCR Protocol
  
• PCR Purification and Storage
  
• Printing in DMSO
  
• Amino-allyl Labeling of RNA for Microarrays
  
• Labeled Probe Hybridization
  
• RNA Extraction from Human/Mouse Cell Lines
  
• RNA Extraction from Human or Animal Tissue Samples
  
• Microarray Slide Staining Procedure
  
• Creating a Microarray Study Binder
  
• High Throughput AminoAllyl Labelling
  
• Amplification of Orientation-specific RNA
  
  also available in PDF format:
  
• Biotechniques (2000), 29(3):548-562
  Hegde P, Qi R, Abernathy R, Gay C, Dharap S, Gaspard R, Earle-Hughes J, Snesrud E, Lee NH, and Quackenbush J

Individual Study Protocols

The individual study protocols are available on the Study Browser pages.

• Mouse Studies
• Rat Studies

A Brief Presentation on our PGA