DNA assembly with error correction on a droplet digital microfluidics platform.

You are here

Authors: Khilko Y, Weyman PD, Glass JI, Adams MD, McNeil MA, Griffin PB
Title: DNA assembly with error correction on a droplet digital microfluidics platform.
Citation: BMC biotechnology. 2018-06-01; 18.1: 37.
Abstract:
Custom synthesized DNA is in high demand for synthetic biology applications. However, current technologies to produce these sequences using assembly from DNA oligonucleotides are costly and labor-intensive. The automation and reduced sample volumes afforded by microfluidic technologies could significantly decrease materials and labor costs associated with DNA synthesis. The purpose of this study was to develop a gene assembly protocol utilizing a digital microfluidic device. Toward this goal, we adapted bench-scale oligonucleotide assembly methods followed by enzymatic error correction to the Mondrian™ digital microfluidic platform.
PMID: 29859085